# HCG: Reference Overview and Reconstitution Notes - facts

Canonical page: https://www.preppinpeppers.com/blogs/the-lab/hcg
Type: Lab article

## TL;DR

HCG (human chorionic gonadotropin) is a protein-based gonadotropin hormone studied in reproductive and endocrine research. Not approved by any regulator.

## Facts Index

| Fact ID | Category | Entity | Attribute | Value | Source | Evidence |
|---|---|---|---|---|---|---|
| hcg:title | identity | HCG: Reference Overview and Reconstitution Notes | title | HCG: Reference Overview and Reconstitution Notes | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:published | identity | HCG: Reference Overview and Reconstitution Notes | published | 2026-08-15 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:author | identity | HCG: Reference Overview and Reconstitution Notes | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:topics | identity | HCG: Reference Overview and Reconstitution Notes | topics | FDA-approved or specific-use, research | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:summary | content | HCG: Reference Overview and Reconstitution Notes | summary | HCG (human chorionic gonadotropin) is a protein-based gonadotropin hormone studied in reproductive and endocrine research. Not approved by any regulator. | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-1 | content | HCG: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-2 | content | HCG: Reference Overview and Reconstitution Notes | section | HCG | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-3 | content | HCG: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-4 | content | HCG: Reference Overview and Reconstitution Notes | section | Research context and categorization | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-5 | content | HCG: Reference Overview and Reconstitution Notes | section | Status | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-6 | content | HCG: Reference Overview and Reconstitution Notes | section | Reconstitution notes (general) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-7 | content | HCG: Reference Overview and Reconstitution Notes | section | Dilution and handling notes (compound-specific) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-8 | content | HCG: Reference Overview and Reconstitution Notes | section | Handling and storage | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-9 | content | HCG: Reference Overview and Reconstitution Notes | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-10 | content | HCG: Reference Overview and Reconstitution Notes | section | Tools and supplies | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:section-12 | content | HCG: Reference Overview and Reconstitution Notes | section | What the research community gets wrong about HCG | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/blogs/the-lab/hcg |
| hcg:product-gansulin-metal-peptide-pen | products | HCG: Reference Overview and Reconstitution Notes | references product | Gansulin Pen · Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/products/gansulin-metal-peptide-pen |
| hcg:product-gansulin-3ml-cartridge | products | HCG: Reference Overview and Reconstitution Notes | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.preppinpeppers.com/products/gansulin-3ml-cartridge |
| hcg:source-pmc-ncbi-nlm-nih-gov | source | HCG: Reference Overview and Reconstitution Notes | cites | Cole, Reprod Biol Endocrinol 2009: New discoveries on the biology and detection of human chorionic gonadotropin - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://pmc.ncbi.nlm.nih.gov/articles/PMC2649930/ |
| hcg:source-pmc-ncbi-nlm-nih-gov-2 | source | HCG: Reference Overview and Reconstitution Notes | cites | Broksø Kyhl et al., Clin Transl Sci 2021: First-in-human trial assessing the pharmacokinetic-pharmacodynamic profile of a novel recombinant human chorionic gonadotropin in healthy women and men of reproductive age - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://pmc.ncbi.nlm.nih.gov/articles/PMC8301557/ |
| hcg:source-www-prnewswire-com | source | HCG: Reference Overview and Reconstitution Notes | cites | FDA Consumer Health Information: HCG Diet Products Are Illegal (FDA notice, 2011) - www.prnewswire.com | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.prnewswire.com/news-releases/fda-consumer-health-information-hcg-diet-products-are-illegal-135105098.html |
| hcg:source-pmc-ncbi-nlm-nih-gov-3 | source | HCG: Reference Overview and Reconstitution Notes | cites | Heidegger and Jeschke, Int J Mol Sci 2018: Human Chorionic Gonadotropin (hCG)-An Endocrine, Regulator of Gestation and Cancer - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://pmc.ncbi.nlm.nih.gov/articles/PMC5983709/ |
| hcg:source-www-uniprot-org | source | HCG: Reference Overview and Reconstitution Notes | cites | UniProtKB/Swiss-Prot P0DN86: Choriogonadotropin subunit beta 3 (CGB3, Homo sapiens), UniProt release 2026-03 - www.uniprot.org | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://www.uniprot.org/uniprotkb/P0DN86/entry |
| hcg:source-clinicaltrials-gov | source | HCG: Reference Overview and Reconstitution Notes | cites | ClinicalTrials.gov NCT01786252 (Phase 4, completed, first posted 2013-02-07): Effect of hCG on Receptivity of the Human Endometrium - clinicaltrials.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/hcg) | https://clinicaltrials.gov/study/NCT01786252 |

## FAQ

### What is the chemical structure of HCG?

HCG is a glycoprotein hormone composed of two subunits: an alpha subunit structurally similar to LH and FSH, and a unique beta subunit that defines HCG's distinct receptor-binding identity.

### How is HCG lyophilized powder reconstituted for laboratory use?

Add bacteriostatic water to the vial and swirl gently — never shake. Divide total IU by water volume added to find concentration (e.g., 5,000 IU ÷ 2 mL = 2,500 IU/mL). Refrigerate at 2–8 °C after mixing.

### Is HCG listed on the WADA Prohibited List?

Yes. WADA classifies chorionic gonadotropin (CG) under S2 (peptide hormones, growth factors, related substances and mimetics) and prohibits it in males at all times, both in and out of competition.

---
Built from PreppinPeppers store data and verified sources only. Same content for every visitor. Research and educational use only, not for human or veterinary use.