# PE-22-28: Reference Overview and Reconstitution Notes - facts

Canonical page: https://www.preppinpeppers.com/blogs/the-lab/pe-22-28
Type: Lab article

## TL;DR

PE-22-28 is a short, lab-made peptide (a small chain of amino acids) based on a natural compound called spadin. It blocks a brain protein called the TREK-1

## Facts Index

| Fact ID | Category | Entity | Attribute | Value | Source | Evidence |
|---|---|---|---|---|---|---|
| pe-22-28:title | identity | PE-22-28: Reference Overview and Reconstitution Notes | title | PE-22-28: Reference Overview and Reconstitution Notes | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:published | identity | PE-22-28: Reference Overview and Reconstitution Notes | published | 2026-08-15 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:author | identity | PE-22-28: Reference Overview and Reconstitution Notes | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:topics | identity | PE-22-28: Reference Overview and Reconstitution Notes | topics | research, Research-only | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:summary | content | PE-22-28: Reference Overview and Reconstitution Notes | summary | PE-22-28 is a short, lab-made peptide (a small chain of amino acids) based on a natural compound called spadin. It blocks a brain protein called the TREK-1 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-1 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-2 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | PE-22-28 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-3 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-4 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Research context and categorization | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-5 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Status | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-6 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Reconstitution notes (general) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-7 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Dilution and handling notes (compound-specific) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-8 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Handling and storage | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-9 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-10 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | Tools and supplies | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:section-12 | content | PE-22-28: Reference Overview and Reconstitution Notes | section | What the research community gets wrong about PE-22-28 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/blogs/the-lab/pe-22-28 |
| pe-22-28:product-gansulin-metal-peptide-pen | products | PE-22-28: Reference Overview and Reconstitution Notes | references product | Gansulin Pen · Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/products/gansulin-metal-peptide-pen |
| pe-22-28:product-gansulin-3ml-cartridge | products | PE-22-28: Reference Overview and Reconstitution Notes | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.preppinpeppers.com/products/gansulin-3ml-cartridge |
| pe-22-28:source-pubmed-ncbi-nlm-nih-gov | source | PE-22-28: Reference Overview and Reconstitution Notes | cites | Djillani et al., Front Pharmacol 2017: Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://pubmed.ncbi.nlm.nih.gov/28955242/ |
| pe-22-28:source-pubmed-ncbi-nlm-nih-gov-2 | source | PE-22-28: Reference Overview and Reconstitution Notes | cites | Djillani et al., Pharmacol Ther 2019: Fighting against depression with TREK-1 blockers: Past and future. A focus on spadin - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://pubmed.ncbi.nlm.nih.gov/30291907/ |
| pe-22-28:source-pubmed-ncbi-nlm-nih-gov-3 | source | PE-22-28: Reference Overview and Reconstitution Notes | cites | Mazella et al., PLoS Biol 2010: Spadin, a sortilin-derived peptide, targeting rodent TREK-1 channels: a new concept in the antidepressant drug design - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://pubmed.ncbi.nlm.nih.gov/20405001/ |
| pe-22-28:source-pmc-ncbi-nlm-nih-gov | source | PE-22-28: Reference Overview and Reconstitution Notes | cites | Veyssiere et al., Psychopharmacology (Berl) 2015: Retroinverso analogs of spadin display increased antidepressant effects - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://pmc.ncbi.nlm.nih.gov/articles/PMC4302242/ |
| pe-22-28:source-pmc-ncbi-nlm-nih-gov-2 | source | PE-22-28: Reference Overview and Reconstitution Notes | cites | Borsotto et al., Br J Pharmacol 2015: Targeting two-pore domain K(+) channels TREK-1 and TASK-3 for the treatment of depression: a new therapeutic concept - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://pmc.ncbi.nlm.nih.gov/articles/PMC4301688/ |
| pe-22-28:source-www-uniprot-org | source | PE-22-28: Reference Overview and Reconstitution Notes | cites | UniProtKB/Swiss-Prot O95069: Potassium channel subfamily K member 2 (KCNK2, Homo sapiens), UniProt release 2026-03 - www.uniprot.org | Lab article (https://www.preppinpeppers.com/blogs/the-lab/pe-22-28) | https://www.uniprot.org/uniprotkb/O95069/entry |

## FAQ

### What is PE-22-28 and what does it do in research?

PE-22-28 is a short synthetic peptide derived from spadin that selectively blocks the TREK-1 potassium channel in brain cells. It is studied in cell and rodent models for effects on mood-related behavior, neurogenesis, and neuroprotection.

### Is PE-22-28 listed on the WADA prohibited list?

PE-22-28 is not approved by any regulatory authority for any use and is handled as an investigational research compound. It is not listed by name on the WADA Prohibited List; those with sport-compliance concerns should consult the current official WADA list directly.

### How do you reconstitute PE-22-28 powder for laboratory use?

Add a measured volume of bacteriostatic water to the dry peptide vial. Concentration (mg/mL) equals milligrams in the vial divided by milliliters added. Use a peptide calculator to determine the exact volume for your target concentration.

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