# Stop Losing Peptides to Dead Volume: Prime Your Pen - facts

Canonical page: https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen
Type: Lab article

## TL;DR

Priming a click-dial pen clears trapped air and residual liquid from the cartridge and pathway, so the dialed volume matches the volume actually dispensed.

## Facts Index

| Fact ID | Category | Entity | Attribute | Value | Source | Evidence |
|---|---|---|---|---|---|---|
| stop-losing-peptides-to-dead-volume-prime-your-pen:title | identity | Stop Losing Peptides to Dead Volume: Prime Your Pen | title | Stop Losing Peptides to Dead Volume: Prime Your Pen | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:published | identity | Stop Losing Peptides to Dead Volume: Prime Your Pen | published | 2026-07-26 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:author | identity | Stop Losing Peptides to Dead Volume: Prime Your Pen | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:topics | identity | Stop Losing Peptides to Dead Volume: Prime Your Pen | topics | Guides, how-to, peptide guide | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:summary | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | summary | Priming a click-dial pen clears trapped air and residual liquid from the cartridge and pathway, so the dialed volume matches the volume actually dispensed. | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-1 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | Pen Priming Defined | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-2 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | What Dead Volume Really Is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-3 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | How a Click-Dial Pen Works | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-4 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | Priming and Clearing Dead Volume: The Steps | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-5 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | Why This Small Step Protects Your Work | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-7 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:section-8 | content | Stop Losing Peptides to Dead Volume: Prime Your Pen | section | What the research community gets wrong about priming pens and dead volume | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:product-gansulin-metal-peptide-pen | products | Stop Losing Peptides to Dead Volume: Prime Your Pen | references product | Gansulin Pen · Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/products/gansulin-metal-peptide-pen |
| stop-losing-peptides-to-dead-volume-prime-your-pen:product-gansulin-3ml-cartridge | products | Stop Losing Peptides to Dead Volume: Prime Your Pen | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://www.preppinpeppers.com/products/gansulin-3ml-cartridge |
| stop-losing-peptides-to-dead-volume-prime-your-pen:source-pubmed-ncbi-nlm-nih-gov | source | Stop Losing Peptides to Dead Volume: Prime Your Pen | cites | Bohannon, Postgrad Med 1999: Insulin delivery using pen devices. Simple-to-use tools may help young and old alike - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://pubmed.ncbi.nlm.nih.gov/10560468/ |
| stop-losing-peptides-to-dead-volume-prime-your-pen:source-pmc-ncbi-nlm-nih-gov | source | Stop Losing Peptides to Dead Volume: Prime Your Pen | cites | Jonker et al., Int J Clin Pharmacol Ther 2021: First pre-filled pen device with highly purified human menopausal gonadotropin (HP-hMG, Menopur) in liquid is shown to be bioequivalent to powder for reconstitution - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://pmc.ncbi.nlm.nih.gov/articles/PMC8594316/ |
| stop-losing-peptides-to-dead-volume-prime-your-pen:source-pmc-ncbi-nlm-nih-gov-2 | source | Stop Losing Peptides to Dead Volume: Prime Your Pen | cites | Lingen et al., Endocr Connect 2023: Advantages and disadvantages of connected insulin pens in diabetes management - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/stop-losing-peptides-to-dead-volume-prime-your-pen) | https://pmc.ncbi.nlm.nih.gov/articles/PMC10563601/ |

## FAQ

### What happens if I don't clear dead volume?

Your first draw will deliver less than the target volume, leading to concentration errors in your samples and potentially flawed data.

### Can I use peptide solution for priming?

No, only use diluent like bacteriostatic water. Using peptide solution wastes the compound and may not fully clear air due to its viscosity.

### How often should I re-prime the pen?

Before the first draw of each day, after changing cartridges, or if the pen has been idle long enough for bubbles to form, typically over 24 hours.

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