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    "faq": [
        {
            "q": "What is a syringe filter used for in HPLC?",
            "a": "It removes particles from a prepared sample before it goes into the autosampler vial. That protects tubing, frits and the column inlet from blockage and helps keep runs repeatable."
        },
        {
            "q": "What does a 0.22 µm syringe filter mean?",
            "a": "The membrane pores are rated at 0.22 µm, which is 0.00022 mm or 220 nanometres. Particles larger than that rating are held back; dissolved molecules pass through."
        },
        {
            "q": "What is the difference between a 4mm and a 13mm syringe filter?",
            "a": "The diameter. A 13 mm filter suits larger sample volumes. A 4 mm filter has a smaller housing, so it suits small-volume work where less liquid should stay trapped."
        },
        {
            "q": "Does a syringe filter remove dissolved impurities from a peptide sample?",
            "a": "No. Dissolved molecules pass through the membrane. A 2014 review on peptide-related impurities describes impurities from solid-phase synthesis that the HPLC method, not the filter, must separate."
        },
        {
            "q": "Is a PES syringe filter a good choice for HPLC sample prep?",
            "a": "PES is one membrane option. Whether it suits your work depends on your solvent and sample diluent, so check the filter maker's chemical compatibility chart before filtering."
        },
        {
            "q": "Can I reuse a syringe filter between samples?",
            "a": "No. Filters are single-use. A reused filter carries over leftovers from the first sample and may already be partly clogged, which raises resistance at the plunger."
        },
        {
            "q": "Why does my plunger feel hard to push through the filter?",
            "a": "The membrane is likely loaded with particles, or the filter is too small for the volume. Do not force it. Swap the filter or spin the sample down first."
        }
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}