# TB-500: Reference Overview and Reconstitution Notes - facts

Canonical page: https://www.preppinpeppers.com/blogs/the-lab/tb-500
Type: Lab article

## TL;DR

TB-500 is a man-made heptapeptide, a seven-amino-acid fragment of thymosin beta-4 studied in tissue-repair research. Overview and reconstitution notes.

## Facts Index

| Fact ID | Category | Entity | Attribute | Value | Source | Evidence |
|---|---|---|---|---|---|---|
| tb-500:title | identity | TB-500: Reference Overview and Reconstitution Notes | title | TB-500: Reference Overview and Reconstitution Notes | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:published | identity | TB-500: Reference Overview and Reconstitution Notes | published | 2026-07-10 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:author | identity | TB-500: Reference Overview and Reconstitution Notes | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:topics | identity | TB-500: Reference Overview and Reconstitution Notes | topics | research, Research-only | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:summary | content | TB-500: Reference Overview and Reconstitution Notes | summary | TB-500 is a man-made heptapeptide, a seven-amino-acid fragment of thymosin beta-4 studied in tissue-repair research. Overview and reconstitution notes. | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-1 | content | TB-500: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-2 | content | TB-500: Reference Overview and Reconstitution Notes | section | TB-500 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-3 | content | TB-500: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-4 | content | TB-500: Reference Overview and Reconstitution Notes | section | Research context and categorization | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-5 | content | TB-500: Reference Overview and Reconstitution Notes | section | Status | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-6 | content | TB-500: Reference Overview and Reconstitution Notes | section | Reconstitution notes (general) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-7 | content | TB-500: Reference Overview and Reconstitution Notes | section | Dilution and handling notes (compound-specific) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-8 | content | TB-500: Reference Overview and Reconstitution Notes | section | Handling and storage | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-9 | content | TB-500: Reference Overview and Reconstitution Notes | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-10 | content | TB-500: Reference Overview and Reconstitution Notes | section | Tools and supplies | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:section-12 | content | TB-500: Reference Overview and Reconstitution Notes | section | What the research community gets wrong about TB-500 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/blogs/the-lab/tb-500 |
| tb-500:product-gansulin-metal-peptide-pen | products | TB-500: Reference Overview and Reconstitution Notes | references product | Gansulin Pen · Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/products/gansulin-metal-peptide-pen |
| tb-500:product-gansulin-3ml-cartridge | products | TB-500: Reference Overview and Reconstitution Notes | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.preppinpeppers.com/products/gansulin-3ml-cartridge |
| tb-500:source-pubchem-ncbi-nlm-nih-gov | source | TB-500: Reference Overview and Reconstitution Notes | cites | NCBI PubChem CID 62707662: TB 500 acetate (C38H68N10O14) - pubchem.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://pubchem.ncbi.nlm.nih.gov/compound/62707662 |
| tb-500:source-www-uniprot-org | source | TB-500: Reference Overview and Reconstitution Notes | cites | UniProtKB P62328: Thymosin beta-4 (TMSB4X, Homo sapiens), UniProt release 2026-03 - www.uniprot.org | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://www.uniprot.org/uniprotkb/P62328/entry |
| tb-500:source-pubmed-ncbi-nlm-nih-gov | source | TB-500: Reference Overview and Reconstitution Notes | cites | Sun and Yin, Ann N Y Acad Sci 2007: The beta-thymosin enigma - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://pubmed.ncbi.nlm.nih.gov/17495248/ |
| tb-500:source-pmc-ncbi-nlm-nih-gov | source | TB-500: Reference Overview and Reconstitution Notes | cites | Zeng et al., Stem Cell Reports 2025: Thymosin beta 4 as an Alzheimer disease intervention target identified using human brain organoids - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://pmc.ncbi.nlm.nih.gov/articles/PMC12447315/ |
| tb-500:source-pubmed-ncbi-nlm-nih-gov-2 | source | TB-500: Reference Overview and Reconstitution Notes | cites | Mayfield et al., Am J Sports Med 2026: Injectable Peptide Therapy: A Primer for Orthopaedic and Sports Medicine Physicians - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://pubmed.ncbi.nlm.nih.gov/41476424/ |
| tb-500:source-clinicaltrials-gov | source | TB-500: Reference Overview and Reconstitution Notes | cites | ClinicalTrials.gov NCT07487363 (Phase 1, Phase 2, recruiting, first posted 2026-03-23): TB-500 (Thymosin Beta 4 17-23 Fragment) for Cardiovascular Biomarkers in Stable ASCVD - clinicaltrials.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/tb-500) | https://clinicaltrials.gov/study/NCT07487363 |

## FAQ

### What is the difference between TB-500 and thymosin beta-4?

Thymosin beta-4 is a full 43-amino-acid protein; TB-500 is a synthetic 7-amino-acid fragment (residues 17–23) derived from it. The two are chemically distinct, though product listings often conflate them.

### How do you reconstitute TB-500 lyophilized powder for lab use?

Add bacteriostatic water to the vial and divide mg of peptide by mL of water added to get concentration. For example, 5 mg + 2 mL = 2.5 mg/mL. Swirl gently; do not shake.

### Is TB-500 banned in sport?

Yes. WADA lists thymosin beta-4 and related fragments, including TB-500, on its Prohibited List under growth factors and growth factor modulators, banned at all times in and out of competition.

---
Built from PreppinPeppers store data and verified sources only. Same content for every visitor. Research and educational use only, not for human or veterinary use.