# Thymosin Alpha-1: Reference Overview and Reconstitution Notes - facts

Canonical page: https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1
Type: Lab article

## TL;DR

Thymosin Alpha-1 is a 28-amino-acid immune-related thymic peptide, approved as a pharmaceutical in some countries but not FDA-approved in the United States.

## Facts Index

| Fact ID | Category | Entity | Attribute | Value | Source | Evidence |
|---|---|---|---|---|---|---|
| thymosin-alpha-1:title | identity | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | title | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:published | identity | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | published | 2026-08-15 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:author | identity | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:topics | identity | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | topics | research, Research-only | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:summary | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | summary | Thymosin Alpha-1 is a 28-amino-acid immune-related thymic peptide, approved as a pharmaceutical in some countries but not FDA-approved in the United States. | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-1 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-2 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-3 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Research context and categorization | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-4 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Status | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-5 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Reconstitution notes (general) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-6 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Dilution and handling notes (compound-specific) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-7 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Handling and storage | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-8 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-9 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | Tools and supplies | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:section-11 | content | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | section | What the research community gets wrong about Thymosin Alpha-1 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1 |
| thymosin-alpha-1:product-gansulin-metal-peptide-pen | products | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | references product | Gansulin Pen · Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/products/gansulin-metal-peptide-pen |
| thymosin-alpha-1:product-gansulin-3ml-cartridge | products | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://www.preppinpeppers.com/products/gansulin-3ml-cartridge |
| thymosin-alpha-1:source-pubchem-ncbi-nlm-nih-gov | source | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | cites | NCBI PubChem CID 16130571: Thymalfasin (C129H215N33O55) - pubchem.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://pubchem.ncbi.nlm.nih.gov/compound/16130571 |
| thymosin-alpha-1:source-pubmed-ncbi-nlm-nih-gov | source | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | cites | Dominari et al., World J Virol 2020: Thymosin alpha 1: A comprehensive review of the literature - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://pubmed.ncbi.nlm.nih.gov/33362999/ |
| thymosin-alpha-1:source-pubmed-ncbi-nlm-nih-gov-2 | source | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | cites | King and Tuthill, Vitam Horm 2016: Immune Modulation with Thymosin Alpha 1 Treatment - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://pubmed.ncbi.nlm.nih.gov/27450734/ |
| thymosin-alpha-1:source-pubmed-ncbi-nlm-nih-gov-3 | source | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | cites | Ancell et al., Am J Health Syst Pharm 2001: Thymosin alpha-1 - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://pubmed.ncbi.nlm.nih.gov/11381492/ |
| thymosin-alpha-1:source-pmc-ncbi-nlm-nih-gov | source | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | cites | Li et al., Peptides 2010: Thymosin alpha 1: biological activities, applications and genetic engineering production - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://pmc.ncbi.nlm.nih.gov/articles/PMC7115394/ |
| thymosin-alpha-1:source-pubmed-ncbi-nlm-nih-gov-4 | source | Thymosin Alpha-1: Reference Overview and Reconstitution Notes | cites | Sjogren, J Gastroenterol Hepatol 2004: Thymalfasin: an immune system enhancer for the treatment of liver disease - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/thymosin-alpha-1) | https://pubmed.ncbi.nlm.nih.gov/15546253/ |

## FAQ

### What is Thymosin Alpha-1 and what is it studied for in research?

A synthetic 28-amino-acid thymic peptide first isolated from thymosin fraction 5. Research examines immune modulation and T-cell maturation. Approved as Zadaxin in roughly three dozen countries; not FDA-approved in the United States.

### Is Thymosin Alpha-1 banned by WADA or prohibited in competitive sports?

It is not specifically listed on the WADA Prohibited List, unlike Thymosin Beta-4/TB-500. Athletes should confirm current status with their governing body, as the S0 non-approved-substances provision can apply to unlisted compounds.

### How do you calculate concentration when reconstituting Thymosin Alpha-1 powder?

Divide the milligrams in the vial by the milliliters of bacteriostatic water added. Example: a 5 mg vial plus 2.5 mL of bacteriostatic water yields a 2 mg/mL solution.

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