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            "value": "The pyrrolizidine moiety and the enzyme that sculpts it",
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            "value": "Five successes, then the wall",
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            "q": "Why does NRPS engineering fail to produce large libraries of peptide variants?",
            "a": "The thioesterase (TE) domain at the assembly-line terminus enforces a strict size limit: peptides with more than two residues upstream of the pyrrolizidine group remain tethered and are never released, halting production regardless of upstream changes."
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            "q": "What is the role of the thioesterase domain in an NRPS assembly line?",
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            "q": "How do bacteria build pyrrolizidine alkaloid moieties in NRPS peptides?",
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                        "text": "The thioesterase (TE) domain at the assembly-line terminus enforces a strict size limit: peptides with more than two residues upstream of the pyrrolizidine group remain tethered and are never released, halting production regardless of upstream changes."
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