# Your peptide pen is losing volume on every click - facts

Canonical page: https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click
Type: Lab article

## TL;DR

A peptide pen's dial number is only as reliable as the tolerances at each mechanical joint, so prime it and check dialed volume against delivered volume.

## Facts Index

| Fact ID | Category | Entity | Attribute | Value | Source | Evidence |
|---|---|---|---|---|---|---|
| your-peptide-pen-is-losing-volume-on-every-click:title | identity | Your peptide pen is losing volume on every click | title | Your peptide pen is losing volume on every click | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:published | identity | Your peptide pen is losing volume on every click | published | 2026-08-15 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:author | identity | Your peptide pen is losing volume on every click | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:topics | identity | Your peptide pen is losing volume on every click | topics | pen peptide usa, peptide pen, reconstitution, research | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:summary | content | Your peptide pen is losing volume on every click | summary | A peptide pen's dial number is only as reliable as the tolerances at each mechanical joint, so prime it and check dialed volume against delivered volume. | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-1 | content | Your peptide pen is losing volume on every click | section | What a peptide pen is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-2 | content | Your peptide pen is losing volume on every click | section | Metal vs Plastic Barrel: The Difference That Shows in Your Data | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-3 | content | Your peptide pen is losing volume on every click | section | Glass Cartridge Tolerances and What They Mean for Reproducibility | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-4 | content | Your peptide pen is losing volume on every click | section | Dead Volume: The Volume You Dial That Goes Nowhere | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-5 | content | Your peptide pen is losing volume on every click | section | Diluent Choice and Practical USA Sourcing Notes | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-7 | content | Your peptide pen is losing volume on every click | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:section-8 | content | Your peptide pen is losing volume on every click | section | What the research community gets wrong about peptide reconstitution pens | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click |
| your-peptide-pen-is-losing-volume-on-every-click:source-pubmed-ncbi-nlm-nih-gov | source | Your peptide pen is losing volume on every click | cites | Jiang et al. 2013 - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pubmed.ncbi.nlm.nih.gov/23872443/ |
| your-peptide-pen-is-losing-volume-on-every-click:source-pmc-ncbi-nlm-nih-gov | source | Your peptide pen is losing volume on every click | cites | Goebel-Stengel et al. 2011 - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pmc.ncbi.nlm.nih.gov/articles/PMC3290000/ |
| your-peptide-pen-is-losing-volume-on-every-click:source-pubmed-ncbi-nlm-nih-gov-2 | source | Your peptide pen is losing volume on every click | cites | Grohganz et al. 2004 - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pubmed.ncbi.nlm.nih.gov/14757490/ |
| your-peptide-pen-is-losing-volume-on-every-click:product-gansulin-metal-peptide-pen | products | Your peptide pen is losing volume on every click | references product | Gansulin Pen · Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/products/gansulin-metal-peptide-pen |
| your-peptide-pen-is-losing-volume-on-every-click:product-gansulin-3ml-cartridge | products | Your peptide pen is losing volume on every click | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://www.preppinpeppers.com/products/gansulin-3ml-cartridge |
| your-peptide-pen-is-losing-volume-on-every-click:source-pubchem-ncbi-nlm-nih-gov | source | Your peptide pen is losing volume on every click | cites | NCBI PubChem CID 244: Benzyl Alcohol (C7H8O) - pubchem.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pubchem.ncbi.nlm.nih.gov/compound/244 |
| your-peptide-pen-is-losing-volume-on-every-click:source-pubmed-ncbi-nlm-nih-gov-3 | source | Your peptide pen is losing volume on every click | cites | Bohannon, Postgrad Med 1999: Insulin delivery using pen devices. Simple-to-use tools may help young and old alike - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pubmed.ncbi.nlm.nih.gov/10560468/ |
| your-peptide-pen-is-losing-volume-on-every-click:source-pmc-ncbi-nlm-nih-gov-2 | source | Your peptide pen is losing volume on every click | cites | Jonker et al., Int J Clin Pharmacol Ther 2021: First pre-filled pen device with highly purified human menopausal gonadotropin (HP-hMG, Menopur) in liquid is shown to be bioequivalent to powder for reconstitution - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pmc.ncbi.nlm.nih.gov/articles/PMC8594316/ |
| your-peptide-pen-is-losing-volume-on-every-click:source-pmc-ncbi-nlm-nih-gov-3 | source | Your peptide pen is losing volume on every click | cites | Lingen et al., Endocr Connect 2023: Advantages and disadvantages of connected insulin pens in diabetes management - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://pmc.ncbi.nlm.nih.gov/articles/PMC10563601/ |
| your-peptide-pen-is-losing-volume-on-every-click:source-dailymed-nlm-nih-gov | source | Your peptide pen is losing volume on every click | cites | Bacteriostatic Water for Injection, USP: FDA label on DailyMed, U.S. National Library of Medicine (0.9% benzyl alcohol) - dailymed.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/your-peptide-pen-is-losing-volume-on-every-click) | https://dailymed.nlm.nih.gov/dailymed/lookup.cfm?setid=87d6e9dc-fe3b-4593-ac9a-d7493d1959c7 |

## FAQ

### What is dead volume in a peptide pen and how do I correct for it?

Dead volume is liquid trapped in the tip and needle hub that never gets delivered. Prime the pen before use to fill the dead space, and verify actual delivered volume with a balance and calibrated calculation on first use of any new pen or cartridge lot.

### Why use bacteriostatic water instead of sterile water for reconstituting peptides?

Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits microbial growth in vials that are punctured multiple times. Sterile water without benzyl alcohol is only appropriate for single-use, single-draw reconstitutions.

### What glass type should a peptide pen cartridge be made from?

Type I borosilicate glass is the pharmaceutical standard. It is chemically inert and will not react with bacteriostatic water or peptide solutions. Always confirm glass type with your supplier before use.

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