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Humanin: Reference Overview and Reconstitution Notes
Machine-readable facts for this Lab article. The full page is https://www.preppinpeppers.com/blogs/the-lab/humanin.
TL;DR
Humanin is a 24-amino-acid peptide encoded in mitochondrial DNA, studied in longevity, metabolic and neuroprotection research. Not FDA-approved; lab notes.
Facts Index
| Fact ID | Category | Entity | Attribute | Value | Source |
|---|---|---|---|---|---|
| humanin:title | identity | Humanin: Reference Overview and Reconstitution Notes | title | Humanin: Reference Overview and Reconstitution Notes | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:published | identity | Humanin: Reference Overview and Reconstitution Notes | published | 2026-08-15 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:last-reviewed | identity | Humanin: Reference Overview and Reconstitution Notes | last reviewed | 2026-10-03 | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:author | identity | Humanin: Reference Overview and Reconstitution Notes | author | Preppin' Peppers Editorial Team | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:topics | identity | Humanin: Reference Overview and Reconstitution Notes | topics | research, Research-only | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:summary | content | Humanin: Reference Overview and Reconstitution Notes | summary | Humanin is a 24-amino-acid peptide encoded in mitochondrial DNA, studied in longevity, metabolic and neuroprotection research. Not FDA-approved; lab notes. | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-1 | content | Humanin: Reference Overview and Reconstitution Notes | section | What Humanin Is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-2 | content | Humanin: Reference Overview and Reconstitution Notes | section | Humanin | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-3 | content | Humanin: Reference Overview and Reconstitution Notes | section | What it is | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-4 | content | Humanin: Reference Overview and Reconstitution Notes | section | Research context and categorization | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-5 | content | Humanin: Reference Overview and Reconstitution Notes | section | Status | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-6 | content | Humanin: Reference Overview and Reconstitution Notes | section | Reconstitution notes (general) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-7 | content | Humanin: Reference Overview and Reconstitution Notes | section | Dilution and handling notes (compound-specific) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-8 | content | Humanin: Reference Overview and Reconstitution Notes | section | Handling and storage | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-9 | content | Humanin: Reference Overview and Reconstitution Notes | section | Related reading | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-10 | content | Humanin: Reference Overview and Reconstitution Notes | section | Tools and supplies | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:section-12 | content | Humanin: Reference Overview and Reconstitution Notes | section | What the research community gets wrong about Humanin | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:product-gansulin-metal-peptide-pen | products | Humanin: Reference Overview and Reconstitution Notes | references product | Gansulin Pen ยท Reusable Click-Dial, 3 ml (1-60 Units) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:product-gansulin-3ml-cartridge | products | Humanin: Reference Overview and Reconstitution Notes | references product | Gansulin-Compatible 3 ml Glass Cartridge for Pens (10-Pack) | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:source-pmc-ncbi-nlm-nih-gov | source | Humanin: Reference Overview and Reconstitution Notes | cites | Woodhead et al., J Appl Physiol (1985) 2020: High-intensity interval exercise increases humanin, a mitochondrial encoded peptide, in the plasma and muscle of men - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:source-pubmed-ncbi-nlm-nih-gov | source | Humanin: Reference Overview and Reconstitution Notes | cites | Zuccato et al., Expert Opin Ther Targets 2019: Mitochondrial-derived peptide humanin as therapeutic target in cancer and degenerative diseases - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:source-pubmed-ncbi-nlm-nih-gov-2 | source | Humanin: Reference Overview and Reconstitution Notes | cites | Voigt and Jelinek, Physiol Rep 2016: Humanin: a mitochondrial signaling peptide as a biomarker for impaired fasting glucose-related oxidative stress - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:source-pmc-ncbi-nlm-nih-gov-2 | source | Humanin: Reference Overview and Reconstitution Notes | cites | Gong et al., Front Endocrinol (Lausanne) 2014: Humanin and age-related diseases: a new link? - pmc.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:source-pubmed-ncbi-nlm-nih-gov-3 | source | Humanin: Reference Overview and Reconstitution Notes | cites | Rochette, Ann Cardiol Angeiol (Paris) 2020: Humanin: A mitochondria-derived peptide with emerging properties - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
| humanin:source-pubmed-ncbi-nlm-nih-gov-4 | source | Humanin: Reference Overview and Reconstitution Notes | cites | Lei and Rao, Biochim Biophys Acta Gen Subj 2022: The role of humanin in the regulation of reproduction - pubmed.ncbi.nlm.nih.gov | Lab article (https://www.preppinpeppers.com/blogs/the-lab/humanin) (evidence) |
FAQ
- What is Humanin peptide and where does it come from?
- Humanin is a 24-amino-acid mitochondrial-derived peptide (MDP) encoded by the MT-RNR2 region of mitochondrial DNA. First identified in 2001, it belongs to a class that includes MOTS-c and the SHLP series, and is studied in preclinical aging and neuroprotection research.
- How is a lyophilized Humanin vial reconstituted for laboratory use?
- Divide the vial's milligram content by the volume of bacteriostatic water added to obtain mg/mL concentration. For example, 5 mg dissolved in 2 mL yields 2.5 mg/mL. A peptide reconstitution calculator can determine exact water volumes for any target concentration.
- Is Humanin listed on the WADA Prohibited List?
- Humanin is not named explicitly on the WADA Prohibited List. However, WADA category S2 (Peptide Hormones, Growth Factors, Related Substances and Mimetics) uses broad language. Athletes must confirm current status directly with their sport's anti-doping authority.