Bronchogen
What it is
Bronchogen is a synthetic short-peptide bioregulator, specifically the tetrapeptide Ala-Glu-Asp-Leu (AEDL), with a molecular weight of roughly 446 g/mol. It belongs to the Khavinson family of peptide bioregulators characterized at the St. Petersburg Institute of Bioregulation and Gerontology, and this is a neutral reference entry describing its identity, regulatory status, and laboratory handling only.
Research context and categorization
Bronchogen is grouped with the Khavinson tissue bioregulators, specifically the subset associated with the bronchopulmonary (lung and bronchial) system. In that literature it is commonly discussed in relation to airway epithelial cells and is investigated for how short peptide sequences may interact with gene-promoter regions and influence the expression of genes tied to epithelial structure, mucin and surfactant pathways, and inflammatory signaling. It has been studied in the context of bronchial epithelial cell proliferation and differentiation and airway tissue regeneration in preclinical and cell-based models.
These uses are investigational and not confirmed or approved. Most of the published work originates from Russian preclinical and laboratory sources, and clear human efficacy data from controlled clinical trials is not established in the English-language literature. The research directions described above should not be read as proven benefits.
Status
- Regulatory status: Not FDA-approved. Bronchogen is supplied and handled as a research-only material, and the published work on it sits at the preclinical/investigational level. It is marketed in Russia as a supplement, which does not equate to FDA approval.
- Sport status: Not specifically listed on the WADA Prohibited List by name. Researchers should note that WADA uses open-ended catch-all language for certain classes and updates the list annually, so status should always be confirmed against the current official WADA Prohibited List.
Reconstitution notes (general)
Bronchogen typically ships as a lyophilized (freeze-dried) powder that is reconstituted before laboratory use. Working concentration is a simple ratio: concentration equals the milligrams of peptide in the vial divided by the millilitres of bacteriostatic water added. For example, a 20 mg vial brought up with 2 mL yields 10 mg/mL, and the same vial with 4 mL yields 5 mg/mL. A reconstitution calculator is available at /pages/tools.
Dilution and handling notes (compound-specific)
Bronchogen is commonly sold in 20 mg vials, and a frequently cited approach is adding roughly 2 mL to 4 mL of bacteriostatic water per vial, which places the working concentration in the approximate 5 mg/mL to 10 mg/mL band. The volume chosen is mostly a matter of the concentration a researcher wants to work at rather than a solubility limit, since this small hydrophilic peptide is described as readily water-soluble.
Being a very short four-residue peptide, Bronchogen generally goes into solution readily and clears to a colorless liquid without the persistent gelling, clouding, or precipitation that larger or more hydrophobic peptides can show when concentrated. Because it dissolves easily, aggressive mixing is unnecessary and counterproductive.
A few practical points reduce trouble. Let both the sealed vial and the bacteriostatic water reach ambient laboratory temperature before mixing, which helps the powder dissolve cleanly. Add the water slowly, aiming the stream down the inside wall of the vial rather than directly onto the powder plug. Then swirl gently or roll the vial between the palms and let it rest rather than shaking, since vigorous agitation whips air into the liquid and causes foaming. If the solution is concentrated heavily and shows temporary cloudiness or undissolved particles, giving it more time at room temperature or adding a little more diluent usually resolves it. Keep the material out of direct light, and avoid repeated freeze-thaw cycles by dividing a batch into single-use aliquots when it will not be used quickly.
Handling and storage
Store the reconstituted vial refrigerated at 2 to 8 degrees Celsius and keep it out of light. Wipe the rubber stopper with an alcohol swab before each puncture, and label the vial with the mix date. Observe the general four-week refrigerated window for reconstituted peptide, and discard any vial that turns cloudy or discolored or that shows floaters or particulate.
Related reading
Tools and supplies
- Reconstitution & blend calculators
- Bacteriostatic Water 30 ml
- Gansulin Metal Reusable Pen
- 3 ml Glass Cartridges (10-pack)
- Complete Starter Kit
For laboratory and research reference only. Educational content, not medical, dosing, injection, or therapeutic guidance, and not intended for human or animal use. Any research uses described are investigational and not confirmed or approved benefits. Confirm anything involving health with a licensed professional. References linked above.