Use this free calculator to reconstitute Semaglutide for research: enter the milligrams in your vial and the millilitres of bacteriostatic water, and it returns the concentration in mg/mL, the volume to draw for any target, and the U-100 unit equivalent. The reference data and concentration matrix below are specific to Semaglutide and can be checked by hand or cited.
Semaglutide reference data
| Class | GLP-1 receptor agonist peptide (31-residue, C18 diacid fatty-acyl / albumin-binding side chain) |
|---|---|
| Molecular formula | C187H291N45O59 |
| Molecular weight | 4114 g/mol |
| CAS | 910463-68-2 |
| PubChem CID | 56843331 |
| Typical vial sizes | 3 mg / 5 mg / 10 mg |
| Storage | Lyophilized: 2-8 C sealed and desiccated, out of light (freeze -20 C for extended storage). Reconstituted: 2-8 C, protect from light; typical ~4-week refrigerated working window. |
Concentration matrix for Semaglutide
Each cell = concentration for that vial mass and diluent volume, with mcg per U-100 unit and nmol/mL (from MW 4114). Concentration = vial mg ÷ water mL.
| Vial ↓ / Water → | 1 mL | 2 mL | 3 mL | 5 mL |
|---|---|---|---|---|
| 3 mg | 3.00 mg/mL 30.0 mcg/unit · 729 nmol/mL |
1.50 mg/mL 15.0 mcg/unit · 365 nmol/mL |
1.00 mg/mL 10.0 mcg/unit · 243 nmol/mL |
0.60 mg/mL 6.0 mcg/unit · 146 nmol/mL |
| 5 mg | 5.00 mg/mL 50.0 mcg/unit · 1215 nmol/mL |
2.50 mg/mL 25.0 mcg/unit · 608 nmol/mL |
1.67 mg/mL 16.7 mcg/unit · 405 nmol/mL |
1.00 mg/mL 10.0 mcg/unit · 243 nmol/mL |
| 10 mg | 10.00 mg/mL 100.0 mcg/unit · 2431 nmol/mL |
5.00 mg/mL 50.0 mcg/unit · 1215 nmol/mL |
3.33 mg/mL 33.3 mcg/unit · 810 nmol/mL |
2.00 mg/mL 20.0 mcg/unit · 486 nmol/mL |
Handling & notes
Amphiphilic due to the C18 diacid side chain, so it can be surface-active and foam; dissolves in bacteriostatic water but benefits from slow, swirling reconstitution. Solutions may show mild viscosity at higher concentrations.
All values here are physical-chemistry reference data and fluid-mixing arithmetic for laboratory and research use only. Not dosing, medical, or therapeutic guidance; not intended for human or animal use. Products are sold for research, educational, and demonstration use only.
