Tesamorelin Reconstitution Calculator

Each cell = concentration for that vial mass and diluent volume, with mcg per U-100 unit and nmol/mL (from MW 5136). Concentration = vial mg ÷ water mL.

Tesamorelin generally dissolves easily. Add the diluent slowly, swirl gently, and let the powder go into solution on its own — vigorous shaking causes foaming and is thought to stress the peptide chain. If the solution stays cloudy, shows particles, or simply does not clear, treat it as unusable.

Choosing a volume. The amount of water is a decision about the concentration you want to work with, not a hard solubility limit. Mixing on the more dilute side is often preferred: the compound dissolves cleanly and the larger draw volumes measure more accurately on a U-100 barrel, because a given percentage error in reading the syringe covers fewer micrograms when each unit carries less mass.

Scale relative to the other compounds here. At 5,136 g/mol tesamorelin is the heaviest peptide in this calculator set — about 7.2× the molecular weight of Ipamorelin. That is why its molar column sits so much lower: 5.00 mg/mL of tesamorelin is roughly 974 nmol/mL, while the same 5.00 mg/mL of Ipamorelin is about 7,023 nmol/mL. Identical mass concentration, very different molecule counts.

Storage. Tesamorelin differs from most peptides in this set in its lyophilized storage: 2-8 C sealed and desiccated out of light is the standard condition, with -20 C used for extended storage rather than as the default. Reconstituted material goes to 2-8 C protected from light. Discard any solution that clouds, discolors, or develops particles.

This is a reconstituted freeze-dried powder — not a pre-mixed solution — so every figure on this page depends on the diluent volume actually added, not on the vial label alone.

A 10 mg vial reconstituted with 3 mL of bacteriostatic water:

• Concentration: 10 mg ÷ 3 mL = 3.33 mg/mL
• Per U-100 unit (1 unit = 0.01 mL): 3.33 mg/mL × 0.01 mL = 0.0333 mg = 33.3 mcg per unit
• Molarity: (3.333 ÷ 5,136) × 1,000,000 = 649 nmol/mL
• Total yield: 3.00 mL of solution = 300 U-100 units in the vial

The same 10 mg in 1 mL would read 10.00 mg/mL and 100 mcg per unit across only 100 units — three times the mass per graduation, and correspondingly less room for precise measurement.

How much water should be used to reconstitute tesamorelin?
Solubility does not force the answer; the volume sets the concentration you want to work with. More dilute mixes are often preferred because the compound dissolves cleanly and larger draw volumes read more accurately on a U-100 syringe barrel.

Why is tesamorelin stored at 2-8 C rather than -20 C in lyophilized form?
2-8 C sealed and desiccated out of light is the standard lyophilized condition for tesamorelin, with -20 C reserved for extended storage. Most other peptides in this calculator set default to -20 C for the powder.

Why are the nmol/mL numbers so much lower than for other peptides?
Molarity is mass divided by molecular weight. At 5,136 g/mol tesamorelin is the heaviest compound here, so a given mg/mL represents far fewer molecules. 5.00 mg/mL is about 974 nmol/mL for tesamorelin versus about 7,023 nmol/mL for Ipamorelin.

What does foaming during reconstitution do?
Foam greatly increases the air-liquid interface, where peptide chains unfold and aggregate. Adding the diluent slowly down the vial wall and swirling instead of shaking avoids it.

How is the mcg-per-unit figure derived?
A U-100 syringe unit is 0.01 mL. Multiply the concentration in mg/mL by 0.01 to get milligrams per unit, then by 1,000 for micrograms. At 3.33 mg/mL that is 33.3 mcg per unit.