PNC-27
What it is
PNC-27 is a synthetic chimeric peptide, roughly 27 to 32 amino acids long, that joins a fragment corresponding to residues 12 to 26 of the p53 protein (the HDM-2-binding region) to a membrane-penetrating leader sequence sometimes called a membrane residency peptide. It belongs to the broad family of engineered p53-derived, cell-penetrating research peptides.
Research context and categorization
PNC-27 is generally grouped under oncology and cancer-cell-biology research rather than any wellness, metabolic, or anti-aging category. It is commonly discussed in relation to a proposed mechanism in which it binds to HDM-2 that is mislocalized to the plasma membrane of certain cancer cells and, in laboratory models, is associated with transmembrane pore formation and cell lysis. Because significant HDM-2 has been reported in the membranes of various transformed cell lines but not in untransformed lines, PNC-27 has been investigated for selective activity against cancer cells in vitro. It has also been studied in the context of leukemia and cervical cancer cell models.
These uses are strictly investigational. PNC-27 has not been through human clinical trials, and none of the effects described in laboratory reports are confirmed or approved benefits. All of this remains preclinical research context, not established outcomes.
Status
- Regulatory status: Research-only, not FDA-approved. There is no approved human indication and no established human safety data.
- Sport status: Not specifically listed by name on the WADA Prohibited List. As a non-approved synthetic peptide, a substance of this type would generally fall under the WADA S0 (Non-Approved Substances) catch-all, which covers substances not addressed by any other section and not currently approved for human therapeutic use. Anyone subject to testing should confirm current status against the official WADA Prohibited List.
Reconstitution notes (general)
PNC-27 ships as a lyophilized (freeze-dried) powder that is reconstituted before laboratory use. The resulting concentration equals the milligrams of peptide in the vial divided by the millilitres of bacteriostatic water added. For example, dividing the vial contents by the volume of diluent gives the milligrams-per-millilitre figure. A reconstitution calculator is available at our reconstitution and blend calculators.
Dilution and handling notes (compound-specific)
PNC-27 is supplied in vials commonly in the 2 mg to 30 mg range and is reconstituted with bacteriostatic water. A frequently cited example is adding about 3.0 mL of bacteriostatic water to a 30 mg vial to reach roughly 10 mg/mL, with the exact volume chosen to fit the concentration a protocol requires. As a longer, partly hydrophobic chimeric peptide that carries both p53-derived residues and a membrane-penetrating leader, PNC-27 can be slower and fussier to dissolve than a short, fully hydrophilic peptide, and it is prone to foaming and to aggregation if handled roughly. The common practical measure is to run the water slowly down the interior wall of the vial rather than directly onto the powder, then swirl or gently roll the vial and let it stand without vigorous shaking or vortexing, which helps limit foaming and denaturation. If the solution stays hazy, cloudy, or shows gel-like wisps that will not resolve with continued gentle swirling and a short rest, that points to incomplete solubilization rather than a finished stock, and such material should not be treated as usable. Freeze-thaw cycles should be avoided, since repeated freezing and thawing can degrade the peptide.
Handling and storage
Store the lyophilized powder cold and dry, out of light. Once reconstituted, keep it refrigerated at 2 to 8 C, out of light, and wipe the stopper with alcohol before drawing. Label the vial with the mix date and concentration. A reconstituted refrigerated solution is generally treated as usable for about a 4-week window as an outer bound. Discard the vial if the contents become cloudy or discolored or show floaters.
Related reading
Tools and supplies
- Reconstitution & blend calculators
- Bacteriostatic Water 30 ml
- Gansulin Metal Reusable Pen
- 3 ml Glass Cartridges (10-pack)
- Complete Starter Kit
For laboratory and research reference only. Educational content, not medical, dosing, injection, or therapeutic guidance, and not intended for human or animal use. Any research uses described are investigational and not confirmed or approved benefits. Confirm anything involving health with a licensed professional. References linked above.