PE-22-28
What it is
PE-22-28 is a short synthetic peptide derived from spadin, a naturally occurring fragment of the sortilin propeptide. It is characterized in the research literature as a selective blocker of the TREK-1 (TWIK-related, K2P2.1) two-pore-domain potassium channel, which is expressed in the brain.
Research context and categorization
PE-22-28 is grouped under the neuroscience, mood, and cognition research category, and more specifically among ion-channel-modulating peptides. It was developed as a shortened, more stable analog of spadin, which was first described around 2010 as a natural TREK-1 blocker showing antidepressant-like activity in animal models.
In neutral research terms, PE-22-28 is commonly discussed in relation to the TREK-1 channel as a molecular target, because animals lacking this channel show a depression-resistant profile in laboratory studies. It is investigated for possible effects on mood-related behavior, neurogenesis, and neuroprotection in preclinical (cell and animal) work. Reports also describe it as being studied in the context of relatively fast onset and an extended duration of action compared with the parent spadin peptide.
These uses are investigational only. As of this writing there are no completed, published human efficacy trials, and none of the described effects are confirmed or approved outcomes. Everything above reflects laboratory and preclinical discussion, not established benefits in people.
Status
- Regulatory status: Research-only, not FDA-approved. PE-22-28 is not approved for any medical indication and is handled as an investigational research compound, with published work limited to cell and rodent studies.
- Sport status: Not specifically listed by name on the WADA Prohibited List. TREK-1 channel blockers are not a named class on the current list, and PE-22-28 does not appear among the peptides WADA identifies by name. Because the list uses category language and is updated periodically, anyone with a competitive-sport concern should confirm current status against the official WADA Prohibited List.
Reconstitution notes (general)
Reconstitution concentration is calculated simply: concentration equals the milligrams of peptide in the vial divided by the millilitres of bacteriostatic water added. Adding more water yields a lower concentration per millilitre, and adding less water yields a higher concentration. To work out a specific value for a given vial size, use the calculator at our reconstitution and blend calculators.
Dilution and handling notes (compound-specific)
PE-22-28 is supplied as a lyophilized (freeze-dried) powder, commonly in vials around the 5 mg to 10 mg range, and is reconstituted with bacteriostatic water. Typical laboratory references pair a vial with roughly 1 mL to 3 mL of bacteriostatic water, which for a 10 mg vial lands in a concentration band of about 3 to 10 mg per mL. The choice of water volume is a trade-off: less water gives a more concentrated, lower-volume solution, while more water makes small-volume measurement easier and more precise.
Because the peptide carries several hydrophobic residues, a freshly mixed vial can look faintly hazy at first and may go into solution a little more slowly than a highly water-soluble peptide. The practical approach is to add the water slowly down the inner wall of the vial rather than directly onto the powder, then let the vial rest and swirl or roll it gently until the solution turns clear. Do not shake, since shaking introduces foam and mechanical stress. Letting the powder and diluent reach room temperature before mixing tends to reduce both haze and condensation. If the solution stays cloudy or shows visible particles after gentle mixing and resting, treat that as incomplete dissolution rather than a finished preparation, and a clear solution as the target endpoint.
Handling and storage
Store the reconstituted solution refrigerated at 2 to 8 C and keep it out of direct light. Wipe the rubber stopper with an alcohol swab before each puncture, and label the vial with the mix date so its age is easy to track. Observe the general four-week refrigerated window for a reconstituted vial. Discard the solution if it becomes cloudy after it should have cleared, if it is discolored, or if it shows floaters or particulate matter. Unreconstituted lyophilized powder is typically kept frozen for longer-term storage per the supplier's guidance.
Related reading
Tools and supplies
- Reconstitution & blend calculators
- Bacteriostatic Water 30 ml
- Gansulin Metal Reusable Pen
- 3 ml Glass Cartridges (10-pack)
- Complete Starter Kit
For laboratory and research reference only. Educational content, not medical, dosing, injection, or therapeutic guidance, and not intended for human or animal use. Any research uses described are investigational and not confirmed or approved benefits. Confirm anything involving health with a licensed professional. References linked above.