Research reference tools

Peptide Calculator
Reconstitution, Cycle, Blend & Dilution Tools

Free, no sign-up, nothing saved: reconstitution and dilution math, a multi-peptide blend calculator, and a peptide cycle & supply planner with one-click calendar export. Work in mg or mcg for standard 3 ml cartridges and multi-use vials.

For research and educational use only. Preppin Peppers sells laboratory hardware and materials (reconstitution pens, cartridges, and bacteriostatic water), not peptides or anything for consumption. All content here, including the calculators, is educational and is not medical, veterinary, dosing, or compounding advice. It has not been evaluated by the FDA and is not intended to diagnose, treat, cure, or prevent any condition, or for human or animal use. You are responsible for complying with your local laws, and use is at your own risk.

Peptide Reconstitution Calculator

Enter your vial and the bacteriostatic (or sterile) water you added, then see exactly how many units to draw. Toggle mg or mcg, and hover any ? for help.

Concentration?How concentrated the mixed solution is: milligrams of peptide per millilitre.5.0 mg/ml
Volume to draw?The millilitres you would draw to get your target amount.0.05 ml
Draw on a U-100 scale?The same volume in units on a standard U-100 syringe or pen. 1 unit = 0.01 ml.5 units
Per unit?How much peptide one unit on a U-100 syringe delivers at this concentration.50 mcg
Draws per vial?How many target-size draws one vial holds, rounded down.10

1 unit on a U-100 syringe or pen = 0.01 ml. 1 mg = 1000 mcg. Illustrative fluid math for research reconstitution.

How to reconstitute your vial

Reconstitution is adding liquid, almost always bacteriostatic water, to a freeze-dried (lyophilized) peptide so it can be measured accurately.

  1. Clean both stoppers. Wipe the bacteriostatic water vial and the peptide vial with an alcohol swab before puncturing either one.
  2. Draw the water first. Pull the full amount of bacteriostatic water into the syringe before adding anything to the peptide vial.
  3. Add the water slowly. Aim the stream down the inside wall of the vial instead of blasting the powder directly.
  4. Swirl gently. Roll or swirl the vial until the powder dissolves fully. Never shake it hard.
  5. Refrigerate after mixing. Keep the reconstituted vial cold and away from light unless the label says otherwise.

Get your target amount from a licensed provider. This calculator handles the arithmetic, not the protocol.

How the math works

The peptide dissolves evenly in the water you add, so the concentration is simply the milligrams in the vial divided by the millilitres of water. U-100 syringes are marked in units, and 100 units equal 1 ml. The three formulas this calculator uses:

  • Concentration (mg/ml) = vial mg ÷ water ml
  • Units to draw = (target mcg ÷ (vial mg × 1000)) × water ml × 100
  • Draws per vial = (vial mg × 1000) ÷ target mcg, rounded down

Example: a 5 mg vial reconstituted with 2 ml of bacteriostatic water is 2.5 mg/ml, or 25 mcg per unit. A 250 mcg target is 10 units on a U-100 syringe (0.10 ml), and the vial holds 20 draws.

Peptide Cycle Calculator & Supply Planner

Add a card per peptide with its amount, schedule, and cycle length, then export to your calendar. Hover any ? for help.

Research supply & scheduling math only. This peptide cycle calculator maps a research schedule and its resupply math — the vials and cartridges a planned run needs, reconstitution timing, and what is left over. It is not a dosing schedule, a protocol, or medical or veterinary advice. Obtain any target amounts and administration guidance from a licensed provider.
Imports into Google Calendar, Apple Calendar and Outlook.

Generated in your browser and never stored. For research planning and supply estimation only, not dosing guidance.

DIY Peptide Blend Calculator

Mixing in one cartridge? Combining peptides is a stability question, not just a volume one. Some stay stable together, others degrade faster, and any blend shortens the beyond-use window. If compatibility is unknown, reconstitute separately or keep short-dated blends cold. This tool handles the volumes once you have decided what can share a cartridge.

Combine peptides into one cartridge and see the draw for each. Hover any ? for help.

Solution in cartridge?The combined volume of the reconstituted solutions you draw into the cartridge.
Top-up water?Plain bacteriostatic water added last when Fill cartridge is on. With no top-up this shows the spare room left in the cartridge.
Each draw?The volume of one draw in U-100 units and millilitres. With no top-up it is the blended solution divided by draws; with Fill cartridge it is the cartridge size divided by draws.
Each draw contains

Assumes each peptide dissolves fully and mixes evenly. Shelf life varies by peptide and handling; research planning math only, not dosing, compounding, or storage guidance. Confirm your protocol and storage with a licensed provider.

Pairs with our hardware

Everything these tools are built around

Reusable all-metal pens, standard 3 ml glass cartridges, and USP bacteriostatic water — the hardware and diluent the calculators assume.

Reference & how it works

Reconstitution is the arithmetic of turning a freeze-dried vial into a measured solution: add a known volume of bacteriostatic water to a known mass of compound, and the concentration is simply mass divided by volume. The reference tables below are fixed values you can check by hand or cite — expand any section.

How the peptide reconstitution calculator works

Enter the milligrams in your vial and the millilitres of water, and the calculator returns the concentration in mg/mL, the volume to draw for any target amount, and the equivalent on a U-100 syringe scale (where 1 unit = 0.01 mL). It also shows how many equal portions a vial holds. Everything runs in your browser; nothing is saved or sent anywhere.

Reconstitution concentration reference (mg/mL)

Each cell is the resulting concentration for a given vial mass and diluent volume, with the amount delivered per U-100 unit in parentheses (1 unit = 0.01 mL, 1 mg = 1000 mcg). Concentration = vial mg ÷ water mL.

Reconstitution concentration reference (mg/mL, with mcg per U-100 unit). Fluid-mixing arithmetic for laboratory reference; not a dosing table.
Vial mass ↓ / Water →1 mL2 mL3 mL5 mL
2 mg2.0 (20)1.0 (10)0.67 (6.7)0.4 (4)
5 mg5.0 (50)2.5 (25)1.67 (16.7)1.0 (10)
10 mg10.0 (100)5.0 (50)3.33 (33.3)2.0 (20)
15 mg15.0 (150)7.5 (75)5.0 (50)3.0 (30)
20 mg20.0 (200)10.0 (100)6.67 (66.7)4.0 (40)
30 mg30.0 (300)15.0 (150)10.0 (100)6.0 (60)

U-100 unit → mL → mg conversion reference

On a U-100 graduated syringe, 100 units = 1 mL, so 1 unit = 0.01 mL. The mg columns show how a stated concentration converts to mass per volume — measurement arithmetic, not dose guidance.

Unit-to-volume conversion for U-100 syringes, with mass at four example concentrations. mg = mL × concentration.
UnitsmLmg @1 mg/mLmg @2.5 mg/mLmg @5 mg/mLmg @10 mg/mL
10.010.010.0250.050.1
20.020.020.050.10.2
50.050.050.1250.250.5
100.100.10.250.51.0
200.200.20.51.02.0
500.500.51.252.55.0
1001.001.02.55.010.0

Per-compound reconstitution calculators

Compound-specific references with molecular data (formula, molecular weight, CAS) and a pre-scoped concentration matrix, for common research compounds:

Frequently asked questions

How do you calculate peptide reconstitution concentration?

Concentration equals the milligrams of compound in the vial divided by the millilitres of bacteriostatic water you add. A 10 mg vial reconstituted with 2 mL of water is 10 ÷ 2 = 5 mg/mL. The calculator does this automatically and also converts it to mcg per U-100 unit.

What does 1 unit on a U-100 syringe equal in mL?

On a U-100 graduated syringe, 100 units = 1 mL, so 1 unit = 0.01 mL. The conversion reference table above lists common unit-to-mL-to-mg equivalents at several example concentrations.

How do I convert between mg and mcg?

1 mg = 1000 mcg. The calculator lets you enter a target in either mg or mcg and handles the conversion; the reference tables show mcg-per-unit so you can read a small amount without converting by hand.

How much bacteriostatic water should I add to a vial?

There is no single correct volume — it sets the concentration. More water gives a more dilute, easier-to-measure solution but a larger draw volume; less water gives a more concentrated solution and smaller draws. Some compounds dissolve more cleanly when more dilute. Use the concentration matrix above to see what each vial-and-water combination produces, then pick the concentration you want to work with. Choosing a preparation concentration is a solubility and handling decision.

What is mg/mL and why does it matter?

mg/mL (milligrams per millilitre) is the concentration — how much compound is dissolved in each millilitre of solution. It is the number that lets you translate any target amount into a volume or a number of syringe units, and it is the core output of the reconstitution calculator.

How many portions does one vial hold?

Divide the total mass in the vial by the target amount per portion. A 10 mg (10,000 mcg) vial at a 250 mcg target yields 40 equal portions. The calculator’s “draws per vial” output computes this for you, rounded down.

Is the calculator free, and is anything stored?

Yes — it is completely free, no sign-up, and it runs entirely in your browser. No inputs are saved, sent, or stored anywhere. The reference tables are static and can be checked by hand.

Is there a peptide cycle calculator?

Yes. The cycle and supply planner maps a research schedule across one or more compounds and returns the supply math — how many vials and cartridges a planned run needs, the reconstitution and resupply timing, and what is left over — with a one-click calendar export. It plans research supply and scheduling, not dosing; obtain any target amounts from a licensed provider.

All of the above is fluid-mixing arithmetic for laboratory and research reference only. It is not dosing, medical, or therapeutic guidance, and nothing here is intended for human or animal use.